Because EMT plays an important role in tumor invasion and metastasis during tumor progression, we further evaluated the associated of Cripto-1 expression with biomarkers of the EMT pathway and revealed that Cripto-1 expression was positively correlated with N-cadherin, Vimentin, Fibronectin and Snail expression and negatively correlated with E-cadherin expression in HCC cells. The gene discussed is SNAI1; the disease is hepatocellular carcinoma.