In this scenario, the aim of this study was to describe the dose–response effects on cell survival induced by proton beam irradiation and, for the first time to our knowledge, the radiation-induced GEPs and immunological molecules profiles produced by the MCF10A mammary non-tumorigenic cell line, and MCF7 (not metastatic, luminal, ER+/PR+/HER2−) and MDA-MB-231 (metastatic, basal, triple negative) BC cell lines with different aggressive phenotypes [21], after graded doses of proton irradiation. Here, ERBB2 is linked to breast cancer.