The results of ROC curve indicated that RAD54L could distinguish BC samples from normal tissues best, among all the hub genes (BUB1B: AUC = 0.934; CCNB1: AUC = 0.884; CDK1: AUC = 0.869; ISG15: AUC = 0.908; KIF15: AUC = 0.888; and RAD54L: AUC = 0.951, Figure 6). This evidence concerns the gene ISG15 and breast cancer.