In the present study, we postulated the construct of SCID mice through a mutation in the Rag2 and IL2rg genes using the CRISPR/Cas9 gene editing tool, sought to determine the biological characteristics of the mutated mice by investigation the immune response against the 85B antigen of Mycobacterium tuberculosis, and establishment a human tumor xenograft model in vivo. This evidence concerns the gene RAG2 and neoplasm.