Furthermore, 105 of liver-resident CD8+ T cells from tumor and non-tumor site of HLA-A2 restricted HCC patients (n = 5) were stimulated with IL-35 for 6 h, and were co-cultured in direct or indirect contact with 5 × 105 of HepG2 cells in the presence of AFP peptide. Here, AFP is linked to hepatocellular carcinoma.