To determine whether Tregs and Th2 cells-mediated immunosuppressed microenvironment by secreting TGF-β and IL-4 in the ischemic hemisphere is directly involved in hyperforin-mediated promotion of neuroangiogenesis and functional recovery after ischemic stroke, blockade of IL-4 and TGF-β1 with neutralizing antibodies was performed. This evidence concerns the gene TGFB1 and ischemic stroke.