C3 and glomerulonephritis: In PBS-treated controls, MRL/lpr mice-derived hybridoma, clone 2B11.3, used in this study caused IgG and C3 deposition along the capillary wall as determined by immunofluorescence at 14 days after hybridoma injection, despite the absence of any apparent features of proliferative glomerulonephritis (NEP25/hybridoma/PBS) (Fig. 2b).