To determine if coinfection with ZIKV and DENV was accompanied by release of cytokines, we quantified plasma cytokine levels using the Cytokine Monkey Magnetic 29-Plex Panel for LuminexTM Platform (Thermofisher Scientific, Waltham, MA) that simultaneously quantifies 29 cytokines (FGF-basic, IL-1β, G-CSF, IL-10, IL-6, IL-12, RANTES, Eotaxin, IL-17, MIP-1α, GM-CSF, MIP-1β, MCP-1, IL-15, EGF, IL-5, HGF, VEGF, IFNγ, MDC, I-TAC, MIF, IL-1RA, TNFα, IL-2, IP-10, MIG, IL-4 and IL-8) and compared them to pre-infection values (Fig. 4a–j and Suppl. This evidence concerns the gene TNF and infection.