As AIF affects the onset and early stages in tumor development, we isolated primary pneumocytes from Aiffl/yKrasG12D and Aif+/yKrasG12D littermates and subsequently infected the cultured cells with Ad5-CMV-Cre-eGFP to activate KrasG12D expression and concomitantly delete Aif (Fig. 2a; Supplementary information, Fig. S3a). Here, AIFM1 is linked to neoplasm.