To further investigate the functional relevance of AIF and AIF-regulated OXPHOS in tumor cell proliferation as well as tumor stem-like properties, we employed a recently developed 3D tumor spheroid culture assay19 with purified primary pneumocytes isolated from Aif+/yKrasG12D, Aiffl/yKrasG12D, Aiffl/yWT ki KrasG12D, Aiffl/yMT ki KrasG12D mice 6 weeks after Ad5-mSPC-Cre infection. This evidence concerns the gene AIFM1 and neoplasm.