CHCHD4 and non-small cell lung carcinoma: Stable knockdown of AIF in KRAS-mutant human NSCLC A549 cells compromised the expression of CHCHD4, a mitochondrial AIF interactor that is important for respiratory chain biogenesis,8 resulting in the reduction of proteins from the respiratory chain such as CIII-UQCRC2, CIV-COXII, and CI-NDUFB8 (Fig. 4a; Supplementary information, Fig. S4), OXPHOS, ATP generation (Fig. 4b, c), clonogenic potential, as well as proliferation (Fig. 4d, e).