Among the nine genes related to body weight changes and the top 20 CpG sites associated with obesity status, the methylation levels of the myomesin 1 (MYOM1), transmembrane protein 48 (TMEM48), and heat shock transcription factor 2 binding protein (HSF2BP) genes were associated with the anthropometric and biochemical features (Fig. 3). This evidence concerns the gene NDC1 and obesity due to melanocortin 4 receptor deficiency.