Female, age < 40 years, body mass index (BMI) < 24 kg/m2, compensated cirrhotic basis, AST-to-Platelet Ratio Index (APRI) < 0.5, no ascites, tumor size≤20 mm, alanine aminotransferase (ALT) ≤40 IU/L, AST ≤40 IU/L, prothrombin time (PT) ≤13 s, ALP ≤110 IU/L, total bile acid (TBA) ≤10 μmol/L, albumin-bilirubin (ALBI) grade 1, PLT ≥100*10^9/L, alpha-fetoprotein (AFP) ≤20 ng/ml, Child-Pugh A and no-touch RFA were set as reference. This evidence concerns the gene AFP and neoplasm.