Then, we validated that YTHDF1 is overexpressed at the mRNA level in 30 primary CRC tissue compared with adjacent normal tissue (Figure 1B and Figure S1) To further validate the expression level of YTHDF1, we detected the expression of YTHDF1 protein in 15 colon cancers and adjacent tissues by immunohistochemistry (IHC). Here, YTHDF1 is linked to colonic neoplasm.