IL4 and neoplasm: To do this, we first used the human monocytic cell line THP-1 to establish M1 macrophage and M2 macrophage models by differentiating the cells with PMA and consequently polarised the differentiated macrophages with LPS plus IFN-γ or IL-4 plus IL-13 respectively.35,36 Identifications of morphological phenotypes, gene expression genotypes and macrophage marker expressions demonstrated that the THP-1-derived M1 and M2 macrophages resemble to tumour-associated M1 and M2 macrophages respectively in multiple aspects.