TNF and metabolic dysfunction-associated steatotic liver disease: First, we assessed mRNA expression of cytokines in the brain of APP/PS1 mice: Tnfα mRNA expression was 38% higher (p < 0.05) in the brain of mice with NAFLD compared to control mice (Figure 4A), with no changes in Il6, Il1β and Tgfβ expressions (Figure 4A).