Expression of typical seminoma markers (PRDM1/BLIMP1, SOX17, PRAME, TFAP2C) was also detectable in TCam-2-ΔSOX2/FOXA2 tumor tissues, in contrast to EC reprogramming factors (GDF3, DPPA3, DNMT3B, GAL), which could only be detected in 2102EP in vivo or reprogrammed TCam-2 cells (Figure 4). Here, PRAME is linked to seminoma.