The infection results showed that EDL933(ΔTir + Y490F) and EDL933(ΔTir + Y519F) induced much higher production of Tnf, Il6 and Il12b than the EDL933(ΔTir + HA-Tir) strain in mouse primary peritoneal macrophages, and EDL933(ΔTir + Y490F, Y519F) induced even higher cytokine production (Figure 5 g). This evidence concerns the gene TNF and infection.