siRNA-mediated knockdown of dectin-1, dectin-2, mincle, MyD88, TRIF, TRAM, MAL, TRAF6, DC-SIGN, NOD1, NOD2, TLR1 or TLR6 had no significant effect on the ability of C. albicans to induce c-Fos expression or MKP1 phosphorylation at 2 h post infection (p.i.)(previously determined to be the optimal time for activation2,21), indicating their collective lack of involvement in the candidalysin response pathway (Supplementary Fig. 1). This evidence concerns the gene NOD1 and infection.