BECN1 and malignant colon neoplasm: Surprisingly, knockdown of Beclin-1 by small interfering RNA (siRNA) significantly inhibited the autophagy activation induced by rapamycin, consequentially suppressing EMT and the invasiveness of colon cancer cells via promoting vimentin and Twist down-regulation and E-cadherin up-regulation, suggesting that inhibiting Beclin-1-induced autophagy would an effective anticancer strategy [65].