ROS also leads to The increase in cleaved or activated PARP in the co-treatment group signifies target melanoma cells with defects in the double-strand DNA repair mechanism with subsequent cellular Caspases, particularly caspase 3 and 7, cleave the 116-kDa form of PARP-1 to generate an 85- and a 24-kDa fragment, on which we performed luminescence studies using ELISA for estimations of different caspases (caspase 8,9,3/7) [28]. The gene discussed is CASP8; the disease is melanoma.