Furthermore, mutated PIK3CA could regulate TF NKX2-5 and induce the mutation of NKX2-5 via EIF2B5 and CKS1B, and then the mutated NKX2-5 is translocated into the nucleus and is regulated by MIR130A. The target gene NKX2-5 is up-regulated in the nucleus due to DNA methylation, and its overexpression promotes the proliferation of cancer cells to induce the inhibition of cancer cell differentiation during thyroid carcinogenesis. This evidence concerns the gene EIF2B5 and cancer.