Comparisons of the previously developed HCC (HuH-7), CCA (KKU-100, KKU-055, KKU-213) and the newly established cells showed all new cell lines expressed two bile duct markers, CK7, and CK9, similar to the previously established cell lines (KKU-100 and KKU-213); only KKK-D138 expressed GGT. This evidence concerns the gene KRT9 and hepatocellular carcinoma.