Similar as in CRC, melanoma cell lines with a hemizygous loss of POLR2A (i.e., IGR-37, SK-MEL-2) had significantly lower levels of POLR2A mRNA and protein than the POLR2A copy neutral cell lines A375-P and UACC-62 (Fig. 4A,B). The gene discussed is POLR2A; the disease is melanoma.