We first found that 4EBP1 is an essential gene in the SUM-44 cells based on results of a genome-scale shRNA screen, and then found that 4EBP1 targeting reduced proliferation of not only amplicon bearing cells (SUM-44, Cama-1, SUM-52) but also non-amplicon ER+ breast cancer cells as well (MCF7, T47D). Here, EIF4EBP1 is linked to breast cancer.