Formalin-fixed, paraffin-embedded (FFPE) coronal mouse brain sections were analysed by fmIHC (Figure 3), including co-staining with an antibody against the reactive astrocyte marker, glial fibrillary acidic protein (GFAP), to aid segmentation of tumour and brain compartments in the digital images prior to fluorescence analysis (Figure S4). This evidence concerns the gene GFAP and neoplasm.