Among the main signaling cascades elicited downstream of CB1 receptor action, we evaluated the protein levels of Akt, p-Akt (Thr308) and p-Akt (Ser473), observing higher expression of both p-Akt (Thr308)/Akt ratio and p-Akt (Ser473)/Akt ratio in tissue samples of CRC patients with metastasis compared to those from patients without metastasis (Figure 4a–c). Here, AKT1 is linked to colorectal carcinoma.