FLG and dry eye syndrome: Mildner et al., by using HSEs and siRNA for FLG, observed loss of keratohyalin granules in the stratum granulosum (SG) and abnormal LB formation [46], similar to findings reported in HEEs where FLG was knocked down by shRNA [47] or generated with IV patient primary KCs [48,49], and in the epidermis of IV patients [19].