To determine whether the effects of SMI or gene silencing on cell viability of NSCLC cell lines are dependent on induction of apoptosis, we determined if the loss of viability is mediated by Caspase 3/7 and induction of apoptosis in PKM2 depleted NSCLC and normal lung cell lines by Tunel assay. This evidence concerns the gene PKM and non-small cell lung carcinoma.