As shown in Fig 2A, all NSCLC cell lines demonstrated a significant and concentration-dependent decrease in PKM2 mRNA expression after treating either with SMI for 48 hours (P = 0.0023) or PKM2 gene silencing (P = 0.001) compared to untreated (none) or negative control shRNA transfected cells. Here, PKM is linked to non-small cell lung carcinoma.