To determine whether the effects of SMI or gene silencing on cell viability of NSCLC cell lines are dependent on induction of apoptosis, we determined if the loss of viability is mediated by Caspase 3/7 and induction of apoptosis in PKM2 depleted NSCLC and normal lung cell lines by Tunel assay. The gene discussed is CASP3; the disease is non-small cell lung carcinoma.