Our data demonstrated that targeting PKM2 with SMI and shRNA-PKM2 affected cell viability and clonogenicity of NSCLC cells in vitro, which confirmed our results that PKM2 is a growth supportive factor We further investigated the mechanism for decreased cell viability and clonogenicity in PKM2 targeted NSCLC cells. The gene discussed is PKM; the disease is non-small cell lung carcinoma.