To confirm that conditioned media from RbLoTem cells also lead to IL-10 production in vivo, we induced pulmonary inflammation in IL-10-GFP reporter mice using lung house dust mite antigen (HDM) challenges and then intranasally administered conditioned media from either RbLoTem or RbHiTn cells, with untreated HDM mice and resting mice as controls. The gene discussed is IL10; the disease is inflammatory response.