Consistent with the previous report, ASD in Gli1-CreERT2/+; Gata4fl/+ embryos was rescued by Pten downregulation (Fig 3G vs. 3D, 1/20 in Gli1-CreERT2/+; Gata4fl/+;Ptenfl/+ vs. 14/29 in Gli1Cre-ERT2/+;Gata4fl/+, P = 0.0013), but the Gli1-CreERT2/+; Gata4fl/+;Ptenfl/+ embryos displayed DORV, consistent with the incidence rate from Gli1-CreERT2/+; Gata4fl/+ embryos (Fig 3H vs. 3E, 12/29 vs. 6/20, Table 1, P = 0.5495). Here, MAPK3 is linked to atrial septal defect.