Cell cycle regulators p16 and p21 have been shown to inhibit tumor cell proliferation and induce cell cycle arrest21 while epithelial‐mesenchymal transition (EMT) in metastasis is characterized by the decrease of epithelial proteins expression (E‐cadherin, ZO‐1 and claudin‐1) and the increase of mesenchymal proteins such as Vimentin and Fibronectin.22 Here, in our study, we found that the protein levels of p16 and p21 in PRDM5 overexpressed AML cells were decreased. Here, TJP1 is linked to acute myeloid leukemia.