Next, to study the function of GPHR in neurons in vivo, we generated PC- and BC-specific GPHR knock-out mice by crossing GPHR floxed mice with GluD2-Cre mice, in which Cre recombinase was knocked-in into the glutamate receptor δ2 (GluD2) locus (Yamasaki et al., 2011). This evidence concerns the gene GLUD2 and breast cancer.