Subsequently, to detect whether the sensitivity of tumor cells to EE02 was reduced after knocking down EGFR, the cancer cells were treated for 24, 48 or 72 h with increasing concentrations of the inhibitor EE02 (H460: 0–3.0 μM; A549: 0–5.0 μM; and MDA-MB-468: 0–2.5 μM), and CCK-8 assays were then performed. The gene discussed is EGFR; the disease is cancer.