EPS8 and non-small cell lung carcinoma: To further identify the inhibitory effect of EE02 on downstream MAPK/Erk and PI3K/Akt signaling, we measured EGFR, Eps8, Akt, p-Akt, Erk and p-Erk protein expression in MDA-MB-468 and H460 (NSCLC cell line) cells after EE02 treatment for 24 h.