To verify that the antitumor effect of EE02 on cancer cells was related to the EGFR protein, we knocked down EGFR expression in H460, A549 and MDA-MB-468 cells; performed western blotting and gray values analysis; and compared the results with those of the vehicle-treated H460, A549 and MDA-MB-468 cells. The gene discussed is EGFR; the disease is cancer.