We next searched for single genes for which low or high expression might be diagnostic, focusing on ALS-increased DEGs with low MIM/CTL FC estimates (e.g., MCTP2, MGAM, CREB5; Additional file 17D) and ALS-decreased DEGs with high MIM/CTL FC estimates (e.g., LDHB, METTL16, FAM102A; Additional file 17E). The gene discussed is METTL16; the disease is amyotrophic lateral sclerosis.