To assess the capability of the NanoBit eIF4E:eIF4G604–646 system to measure the effect of anti-cancer drug resistance mechanisms on the eIF4F complex status, the effect of MYC overexpression on the potency of BEZ235 was tested by co-transfecting GFP fused v-Myc (referred to as GFP-MYC from now on) alongside the components of the protein complementation system. This evidence concerns the gene EIF4E and cancer.