TNF and colitis: Figure 5A–C shows the alterations in the mRNA expression of proinflammatory biomarkers TNF-α, IL-1β and IL-6 in the colonic mucosa of sedentary or treadmill exercising mice fed a SD or HFD with colitis and the changes in the colonic mucosal tissue content of proinflammatory (TNF-α, IL 1β, IL-6, IL-17, and INFγ) and anti-inflammatory cytokine IL-10 in mice with TNBS colitis fed SD or HFD with or without forced treadmill exercise (Figure 5D–I).