To test if ZAP recruitment to SGs is correlated with SINV infection, the entire imaging period, 24 hours, was quantified by first checking in the hZAP-GFP-expressing cells whether there was evidence for infection (double positive for mCherry and GFP), then following the individual cell back in time to see if hZAP-GFP-containing SGs ever formed in that cell. This evidence concerns the gene ZC3HAV1 and infection.