The C‐terminal domain interacts with the HAND2, NF‐AT3, MEF2C, and NKX2.5 transcription factors which play important roles in the heart development.37 Although two other variants, c.1129A>G and c.1138G>A, were also detected in the conserved regions, in silico and segregation analyses, that is, the present variants in the healthy members of the families, indicated these changes have no effect in CHD manifestation. This evidence concerns the gene HAND2 and coronary artery disorder.