66 CHD patients, numbered as CHD‐1 to CHD‐66 (our information about individuals condition was based on genetic counseling and family report), were recruited in this study in which no previously known CHD‐related pathogenic variant was found in the NKX2‐5, ZIC3, NODAL, FOXH1, and GJA1 genes by PCR‐direct sequencing. The gene discussed is NKX2-5; the disease is coronary artery disorder.