Indeed, in our study, C. perfringens type C infection leaded to the significantly dysregulated expressions of proinflammatory factors (TNFAIP8, IL-1A, IL-1B, IL-4, IL-6, and IL-7), interferon (IFNE and IFNG) and toll-like receptor (TLR6, TLR8, TLR9, and TLR10) of infected piglets, suggesting inflammatory response had occurred in the injury intestine portions during infection. This evidence concerns the gene TLR9 and infection.