Although we identified a TF network associated with Tfh differentiation during SIV infection, this cannot exclude the role of additional TFs reported to contribute to their regulation such as the transcription factor achaete-scute homolog 2 (Ascl2) as well as the inhibitor of DNA-binding 2 (Id2) and Id3100,101 demonstrating the complexity of Tfh regulation in the context of infectious diseases. Here, ASCL2 is linked to infectious disease.