To identify molecular mediators of cell mechanical enhancement upon treatment with TGFβ, we first focused on actin and vimentin filaments found that TGFβ-treatment does not affect the G/F-actin ratio in H1975, H1650 and H2030 NSCLC cells (Fig. 5A), but rather induces overexpression of vimentin, Fig. 5B,C. This evidence concerns the gene VIM and non-small cell lung carcinoma.