In summary, our results provide novel insights into phenotypic and transcriptomic response of NSCLC cells to stimulation with TGFβ and suggest that mediators of elevated cell stiffness and migration activity such as overexpressed vimentin intermediate filaments, components of actin and microtubule cytoskeleton, actomyosin complex, and, in particular, unconventional myosins represent promising pharmaceutical targets for suppressing metastatic dissemination of lung cancer cells. This evidence concerns the gene TGFB1 and non-small cell lung carcinoma.