To confirm our conjecture that TFAP2B acts in thyroid cancer tumorigenesis and development, we knocked down TFAP2B in thyroid cancer cell lines and found that cell viability, clonogenicity, migration, and invasion were reduced in vitro, and the opposite effect occurred after overexpression of TFAP2B in these thyroid cancer cells. The gene discussed is TFAP2B; the disease is thyroid gland carcinoma.