CH25H and steatosis: Histological hallmarks of NASH (steatosis, cellular hypertrophy, and necroinflammation) were detected in most mice after 20 weeks of HFD feeding; however, no differences were noted when knockout mice (Ebi2−/−, Ch25h−/−, and Cyp7b1−/−) and their respective littermate wild-type controls were compared (Fig. 3A, B).