In this study, we showed that the ANXA2-expressing Listeria-based immunotherapy (Lm-ANXA2) prolonged survival in the mouse model of PDACs and the sequential treatment with Lm-ANXA2 followed by anti-PD-1 therapy increased the interferon γ (INFγ) expression of an ANXA2 epitope specific T cell response within the tumor microenvironment. Here, ANXA2 is linked to neoplasm.