Sphere formation (Fig. 3a) and flow cytometry (Fig. 3b) presented with sophisticated sphere formation and CD133-positive rate of A549-SP+ cells compared to the A549-SP− cells (p < 0.05), which in turn supported and aggravated the involvement and impact of A549-SP+ cells in NSCLC CSCs. This evidence concerns the gene TFF2 and non-small cell lung carcinoma.