Following our speculation if lncRNA MBNL1-AS1 could serve as a potential target to affect NSCLC by regulating miR-301b-3p and TGFBR2, with a supplementary aim to calculate the expression rate of lncRNA MBNL1-AS1 in NSCLC tissues; foremost, RT-qPCR was conducted in order to examine the expression of lncRNA MBNL1-AS1 in NSCLC tissues and adjacent normal tissues from 56 patients, tissues with and without lymph node metastasis, NSCLC cell lines (A549, H1299, and SK-MES-1), and human normal lung epithelial cell line BEAS-2B. This evidence concerns the gene MBNL1 and metastatic malignant neoplasm in the lymph nodes.