With the abovementioned literature serving as a hypothesis, we obtained NSCLC tissues and adjacent normal tissues to explore and develop tumorigenicity assay in nude mice in order to verify the conceivable effects of lncRNA MBNL1-AS1 on the cellular processes of NSCLC CSCs via miR-301b-3p and TGFBR2. Here, TGFBR2 is linked to non-small cell lung carcinoma.