The key observations of the current study were primarily centered around the protection of CSC cells from the degrading nature of proliferation, invasion, drug resistance, and sphere formation in NSCLC by activating TGFBR2 via a blockade of the miR-301b-3p upon restoration of lncRNA MBNL1-AS1 expression. This evidence concerns the gene TGFBR2 and non-small cell lung carcinoma.