To investigate the possible roles of Pik3ca and Egfr in pancreatic cancer progression, we used the FC1245 pancreatic cancer cell line that was isolated from a KrasLSL–G12D/+ Trp53LSL–R172H/+ Pdx1-Cre mouse in the C57BL/6 (B6) genetic background (25). Here, PIK3CA is linked to familial pancreatic carcinoma.