MECP2 and Anxiety: Other than a small reduction of walking time in hM3Dq-expressing WT mice (p=0.0296, One-way ANOVA), there were no differences in grooming behavior, locomotion, or anxiety-like behaviors between DREADD-expressing WT and Mecp2 KO mice and their mCherry-expressing controls after CNO treatment (p>0.05; Figure 4—figure supplement 4B–F).