Other than a small increase of walking time and aggressive behaviors in WT mice with hM3Dq expression in the vHIP-mPFC projection (Walk, p=0.0438, Student's t-test; Aggression, p=0.0214, Mann-Whitney Test due to nonparametric distributions), there were no differences in grooming behavior, locomotion, or anxiety-like behaviors between DREADD-expressing WT and Mecp2 KO mice and their mCherry-expressing controls after CNO treatment (p>0.05; Figure 5—figure supplement 1D–H). The gene discussed is MECP2; the disease is Anxiety.