To confirm this sequential order of events in our experimental system, we analyzed the promoters of MIRLET7D targets by ChIP using antibodies specific for acetylated H3K27 (H3K27ac) or trimethylated H3K27 (H3K27me3) and chromatin isolated from IPF fibroblasts after MIRCTRL or MIRLET7D transfection alone or in combination with shRNAs specific for HDAC1 (shHDAC1; Supplementary Fig. 5a) or EZH2 (shEZH2; Supplementary Fig. 5b). Here, HDAC1 is linked to idiopathic pulmonary fibrosis.