Further analysis of MIRLET7D targets by ChIP (Supplementary Fig. 7c), ChIP–reChIP (Fig. 6c), and qRT-PCR (Supplementary Fig. 7d) in Ctrl and IPF fibroblasts, in which two methods of EP300-LOF were used, EP300inh treatment or transfection of dominant-negative EP300 (dnEP300; Supplementary Fig. 7a, b)44, clearly demonstrated the causal involvement of actP300 during HDAC inactivation in the nucleus of IPF fibroblasts. The gene discussed is HDAC9; the disease is idiopathic pulmonary fibrosis.