Univariate analysis for 17 variables including NCALD, age, WBC count, PB-blast, BM-blast and genes mutation (DNMT3A, NPM1, TET2, FLT3, IDH2, IDH1, RUNX1, NRAS, WT1, CEBPA, PTPN11, KRAS, mutation vs. wild type) from 74 TCGA CN-AML patients was conducted (Additional file 1: Table S5). Here, WT1 is linked to acute myeloid leukemia.