Furthermore, to explore the functions of TGIF1 in regulating cancer stem cell (CSC) properties, we compared the expression levels of stemness transcriptional factors, such as Sox2, Nanog, CD44, Nestin, and CD133, between the TGIF1-deficient PDAC cells and TGIF1-sufficient PDAC cells. This evidence concerns the gene TGIF1 and cancer.