Incubation with 1 nM of phorbol 12-myristate 13-acetate (PMA; the prototypic phorbol ester) [8] did not have significant effects in both DLD-1 and DLD-1-PKCα cells, which is in agreement with the low endogenous PKC levels in CRC cells. The gene discussed is PRRT2; the disease is colorectal carcinoma.