These changes were associated with a drastic remodeling of the actin network (Figure 5A, lower panels, phalloidin staining), relocation of actin, E-cadherin and β-catenin into membrane protrusions and large buds (Figure 5A, lower left panels, black and solid arrowheads), decrease of the cell size (Figure 5B), and cell cycle arrest in the G0/G1 phase (Figure 5C), as previously reported in colon cancer cell lines including DLD-1 cells [12,13,24,25,26,27]. Here, CDH1 is linked to malignant colon neoplasm.