To evaluate the effects of sustained PKCα activity on CRC cell growth capacity, we cultured parental DLD-1 and DLD-1-PKCα cells at very low concentration (1000 cells/10 cm dish) in the presence or absence of 1 μg/mL doxycycline and/or 1 nM PMA. The gene discussed is PRKCA; the disease is colorectal carcinoma.